Anti-inflammatory and Nociceptive Properties of the Root Aqueous Extract of Vernonia amygdalina and Ocimum graticimum Using Animal Models

Benjamin Ogunma Gabriel, Marian Jegbefumere Osemwegie, Innocent Inomo Inomo, Ofunre Ozolua Eboreime

Abstract


Vernonia amygdalina is a medicinal plant used in folk medicine for the treatment of several diseases. Ocimum graticimum plant roots act as sedatives for children. Ocimum tea is an infused form of the herb used in treating fever and diaphoresis. The study aims to evaluate the anti-inflammatory and analgesic properties of the root extracts of biherbal using Swiss mice and Wistar rats. The plant samples were collected, air-dried, ground, and processed into a root biherbal extract. Adult Swiss mice and Wistar rats were obtained to assess anti-inflammatory and analgesic effects using animal models. The results obtained showed a significant reduction in edema at 100 and 200 mg/kg of the extract. The analgesic effects at 100 and 200 mg/kg had a significant reduction in acetic acid and formalin-induced pain models. All effects were statistically significant (p < 0.05). The biherbal extract exhibits significant anti-inflammatory and analgesic effects, with further research needed to identify bioactive compounds and explore its modern medical potential.


Keywords


Anti-inflammatory; Antinociceptive; Vernonia amygdalina; Ocimum graticimum; Animal Models

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DOI: https://doi.org/10.14421/biomedich.2026.151.1699-1704

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Copyright (c) 2026 Benjamin Ogunma Gabriel, Marian Jegbefumere Osemwegie, Innocent Inomo Inomo, Ofunre Ozolua Eboreime



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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