Enhancement of Probiotic Viability and Antioxidant Activity in Yogurt Treated with Corn Silk Extract

Dina Tri Marya, Arizki Tri Nopitariyani, Gusma Gama Maradon, Muhammad Hanif

Abstract


Yogurt is a widely consumed fermented milk product known for its numerous health benefits, while corn silk, the pistil of corn, is a byproduct rich in bioactive compounds and antioxidants. This study assessed the antioxidant activity of yogurt fortified with corn silk extract. Yogurt was prepared by fermenting cow's milk with a yogurt starter culture. The antioxidant activity of the resulting yogurt was evaluated using the DPPH method. The results demonstrated that the yogurt enriched with corn silk exhibited a total lactic acid bacteria (LAB) count of 1.2 × 108 cfu/mL, a pH of 4.4, a lactic acid content of 1.34%, and an antioxidant activity of 54%. In contrast, the control yogurt without corn silk had a LAB count of 1.9 × 10ⷠcfu/mL, a pH of 4.5, a lactic acid content of 0.93%, and an antioxidant activity of 47%. Organoleptic evaluations revealed no significant differences in taste, aroma, texture, or overall acceptability between the two yogurt types. The addition of corn silk significantly increased the antioxidant activity, total microbial count, and lactic acid content of the yogurt. These findings suggest that corn silk extract can be a beneficial natural additive to enhance the antioxidant properties of yogurt, thereby improving its nutritional value.

Keywords


Antioxidant activity; cornsilk; DPPH method; fermented milk; lactic acid bacteria (LAB); yogurt

Full Text:

PDF

References


AOAC. (2023a). AOAC Official Method 947.05Acidity of Milk. In Official Methods of Analysis of AOAC International. Oxford University Press. https://doi.org/10.1093/9780197610145.003.3040

AOAC. (2023b). AOAC Official Method 981.12pH of Acidified Foods. In Official Methods of Analysis of AOAC International. Oxford University Press. https://doi.org/10.1093/9780197610145.003.3563

Balakrishnan, G., & Agrawal, R. (2014). Antioxidant activity and fatty acid profile of fermented milk prepared by Pediococcus pentosaceus. Journal of Food Science and Technology, 51(12), 4138–4142. https://doi.org/10.1007/s13197-012-0891-9

Caleja, C., Barros, L., Antonio, A. L., Carocho, M., Oliveira, M. B. P. P., & Ferreira, I. C. F. R. (2016). Fortification of yogurts with different antioxidant preservatives: A comparative study between natural and synthetic additives. Food Chemistry, 210, 262–268. https://doi.org/10.1016/j.foodchem.2016.04.114

Ebrahimzadeh, M. A., Pourmorad, F., & Hafezi, S. (2008). Antioxidant Activities of Iranian Corn Silk. Turkish Journal of Biology, 32(1), 43–49.

El-Khadragy, M. F., Al-Olayan, E. M., Elmallah, M. I. Y., Alharbi, A. M., Yehia, H. M., & Abdel Moneim, A. E. (2019). Probiotics and yogurt modulate oxidative stress and fibrosis in livers of Schistosoma mansoni-infected mice. BMC Complementary and Alternative Medicine, 19(1), 3. https://doi.org/10.1186/s12906-018-2406-3

Gomaa, E. Z. (2020). Human gut microbiota/microbiome in health and diseases: a review. Antonie van Leeuwenhoek, 113(12), 2019–2040. https://doi.org/10.1007/s10482-020-01474-7

Guo, Q., Chen, Z., Santhanam, R. K., Xu, L., Gao, X., Ma, Q., Xue, Z., & Chen, H. (2019). Hypoglycemic effects of polysaccharides from corn silk (Maydis stigma) and their beneficial roles via regulating the PI3K/Akt signaling pathway in L6 skeletal muscle myotubes. International Journal of Biological Macromolecules, 121, 981–988. https://doi.org/10.1016/j.ijbiomac.2018.10.100

Gurkan, H., Hayaloglu, A. A., & Boran, O. S. (2019). Influence of purple basil extract (Ocimum basilicum L.) on chemical composition, rheology and antioxidant activity of set-type yoghurt. Mljekarstvo, 69(1), 42–52. https://doi.org/10.15567/mljekarstvo.2019.0104

Hosseini, S. M., & Behbahani, M. (2021). Enhancement of probiotics viability and lactic acid production in yogurts treated with Prangos ferulaceae and Carum copticum plant extracts. Biocatalysis and Agricultural Biotechnology, 35, 102084. https://doi.org/10.1016/j.bcab.2021.102084

Illiano, P., Brambilla, R., & Parolini, C. (2020). The mutual interplay of gut microbiota, diet and human disease. The FEBS Journal, 287(5), 833–855. https://doi.org/10.1111/febs.15217

Li, P., Huang, Y., Zhu, H., Chen, J., Ren, G., Jiang, D., & Liu, C. (2023). Authentication, chemical profiles analysis, and quality evaluation of corn silk via DNA barcoding and UPLC-LTQ/Orbitrap MS chemical profiling. Food Research International, 167, 112667. https://doi.org/10.1016/j.foodres.2023.112667

Niemann, B., Rohrbach, S., Miller, M. R., Newby, D. E., Fuster, V., & Kovacic, J. C. (2017). Oxidative Stress and Cardiovascular Risk: Obesity, Diabetes, Smoking, and Pollution. Journal of the American College of Cardiology, 70(2), 230–251. https://doi.org/10.1016/j.jacc.2017.05.043

Pereira, E., Barros, L., & Ferreira, I. (2013). Relevance of the Mention of Antioxidant Properties in Yogurt Labels: In Vitro Evaluation and Chromatographic Analysis. Antioxidants, 2(2), 62–76. https://doi.org/10.3390/antiox2020062

Raghuvanshi, R. S., Singh, A., & Apurva. (2024). Exploring nutritional and functional properties of corn silk for comprehending its uses. International Journal of Current Advanced Research, 13(7), 3171–3176. https://doi.org/10.24327/ijcar.2024.3176.1685

Rahman, N. A., & Wan Rosli, W. I. (2014). Nutritional compositions and antioxidative capacity of the silk obtained from immature and mature corn. Journal of King Saud University - Science, 26(2), 119–127. https://doi.org/10.1016/j.jksus.2013.11.002

Wang, Y., Wu, J., Lv, M., Shao, Z., Hungwe, M., Wang, J., Bai, X., Xie, J., Wang, Y., & Geng, W. (2021). Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.612285

Wilkins, L. J., Monga, M., & Miller, A. W. (2019). Defining Dysbiosis for a Cluster of Chronic Diseases. Scientific Reports, 9(1), 12918. https://doi.org/10.1038/s41598-019-49452-y

Zhang, X., Zhang, C., Xiao, L., Zhao, X., Ma, K., Ji, F., Azarpazhooh, E., Ajami, M., Rui, X., & Li, W. (2024). Digestive characteristics of extracellular polysaccharide from Lactiplantibacillus plantarum T1 and its regulation of intestinal microbiota. International Journal of Biological Macromolecules, 259, 129112. https://doi.org/10.1016/j.ijbiomac.2023.129112




DOI: https://doi.org/10.14421/biomedich.2025.141.389-393

Refbacks

  • There are currently no refbacks.




Copyright (c) 2025 Dina Tri Marya, Arizki Tri Nopitariyani, Gusma Gama Maradon, Muhammad Hanif



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC