Evaluation of Antidiabetic Activity of Croton zambesicus Root Extract: In Vivo Inhibitory Effect on Alpha Amylase and Alpha Glucosidase of Rats

Jude Efiom Okokon, Utibe Anietie Edem, Chinyelu Clementina OSIGWE, Ugonma Florence Uwaeme

Abstract


Diabetes mellitus is a global health challenge, necessitating the need for alternative treatments. Croton zambesicus Muell Arg. (Euphorbiaceace) a medicinal plant used traditionally in the treatment of some ailments was investigated for its inhibitory potential on alpha-amylase and alpha-glucosidase enzymes in rats. The root extract of C. zambesicus (27 - 81 mg/kg) was investigated in vivo for the inhibitory effect on alpha amylase and alpha glucosidase enzymes using starch, sucrose, and maltose as substrates. Acarbose was used as a reference drug. Blood glucose levels (BGL) of rats, post administration of the substrate and extract concurrently, were monitored over 3 hours as a parameter to measure the inhibitory potential of the extract. The root extract dose-dependently caused significant (p<0.05) reduction in blood glucose levels of treated rats with the various substrates used. The results suggest that the root extract of Croton zambesicus has the potentials to inhibit alpha amylase and alpha glucosidase in rats.

Keywords


Croton zambesiscus; Anti-diabetic; Enzyme inhibition; Alpha-amylase; Alpha-glucosidase

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References


Adjanohoun EJ, Adjakide V, de Souza S. (1989). Contribution to ethnobotanical and floristic studies in Republic of Benin.Vol.1. Agency for Cultural and Technical Cooperation. pp.245.

Alongi M and Anese M. (2018). Effect of Coffee Roasting on in vitro ?-glucosidase activity: inhibition and Mechanism of Action. Food Res Intl. 111:480-487.

Boyom FF, Keumdjio F, Dongmo PM, Ngadjui BT, Amvam-Zollo PH, Menut C, Bessiere JM. (2002). Essential oil from Croton zambesicus Muell. Arg. growing in Cameroun. Flav Fragr J. 17:215 – 21.

Etuk IC, Ebong N, Okokon JE (2023). Inhibitory effect of leaf extract and fractions of Solanum anomalum on alpha amylase and alpha glucosidase enzymes. Biology Medicine and Natural Product Chemistry.21(1):145-150.

Eweh US, Igwe CJ, Effiong GS, Ebong NO, Okokon JE (2022). Evaluation of inhibitory effect of two common vegetables (Heinsia crinata and Lasianthera africana) on alpha amylase and alpha glucosidase enzymes of rats. Journal of Current Biomedical Research.2(5):405-414.

Gidado A, Watafua M, Sa’ad RS, Tagi HS, Abdullahi S. (2019). Alpha-amylase, maltase and sucrase inhibitions by aqueous leaf extracts of Anacardium occidentale (Anacardiaceae) and Piliostigma reticulatum (Caesalpiniaceae) in rats. Trop J Nat Prod Res, 3(6):210-215.

Kalra S. (2014). Alpha Glucosidase Inhibitors. The J Pak Med Assoc. 64(4):474-476.

Karuppiah Vijayamuthuramalingam UD, Rajaram R, Madhavaram Kuppusamy K, Jonnalagadda B, Arokiasamy S. (2017). Anti-hyperglycemic and antioxidant potential of Croton bonplandianus. Bail fractions in correlation with polyphenol content.Iran J Basic Med Sci 20:1390-1397.

Le HTT, Hioku Y, Danova A, Nguyen VK, Duong TH, Kita M, Chavasiri W. (2023). ?-Glucosidase inhibition of sesquiterpenoids from the heaertwood of Mansonia gagei. Phytochemistry. 2023, 213: 113778.

Morocho V, Sarango D, Cruz-Erazo C, Cumbicus N, Cartuche L, Suárez A. I. (2020). Chemical Constituents of Croton thurifer Kunth as ?-Glucosidase Inhibitors. Rec. Nat. Prod. 14:(1) 31-41.

Okokon JE, Nwokafor I, Ebong NO. (2023a). In vivo inhibitory effect of Panicum maximum root extract on alpha amylase and alpha glucosidase enzymes of rats. Journal of Current Biomedical Research. 3(4):1084-1095.

Okokon JE, Amazu LU, NwiduLLl. (2015). Antidiabetic and hypolipidemic activities of Solenostemon monostachyus. Journal of Herbal Drugs. 6(2):83 - 90

Okokon JE, Antia BS, Osuji L, Udia PM. (2007). Antidiabetic and hypolipidaemic effects of Mammea africana (guttiferae) in streptozotocin induced diabetic rats. Journal of Pharmacology and Toxicology. 2(3): 278-283.

Okokon JE, Antia BS, Udobang JA. (2012). Antidiabetic activities of ethanolic extract and fractions of Anthocleista djalonensis. Asian Pacific Journal of Tropical Biomedicine. 2(6): 461 - 464.

Okokon JE, Antia BS, Umoh EE, Etim EI (2010). Antidiabetic and hypolipidaemic effects of Hippocratea africana (Hippocrateaceae) in streptozotocin induced diabetic rats. Int. Journal of Drug Dev. and Research 2(3): 501 - 506.

Okokon JE, Bassey AL, Nyong EE, Edem UA. (2020). Croton zambesicus root extract exerts laxative effect in rats. Asian Journal of Biology 10(2):22-26.

Okokon JE, Dar A., Choudhary MI. (2013). Immunomodulatory, cytotoxic and antileishmanial activities of phytoconstituents of Croton zambesicus. Phytopharmacology. 4(1): 31 – 40.

Okokon JE, Davies K, Lekara J, Iwara K, Thomas P, Li W-W. (2022a). Antidiabetic and hypolipidemic activities of solvents fractions of Setaria megaphylla and its phytochemical characterisation. Phytomedicine Plus. 2(2022):100182.

Okokon JE, Etuk IC, Thomas P, Drijfhout P, Claridge TM, Li W-W. (2022b). In vivo antihyperglycaemic and antihyperlipidemic activities and chemical constituents of leaf extract and fractions of Solanum anomalum in alloxan-induced diabetic rats. Biomedicine and Pharmacotherapy 151(2022):113153.

Okokon JE, Nwafor PA, Ekpo E, Okokon P, Udobang J. (2011a). Antidiabetic and hypolipidemic activities of ethanolic root extract of Croton zambesicus on alloxan induced diabetic rats. Asian Journal of Pharmaceutical Research 1: 493 – 499.

Okokon JE, Davies K, Lekara J, Iwara K, Kumar H. (2021). Effect of solvents leaf fractions of Setaria megaphylla on alpha amylase and alpha glucosidase activities in rats. Advanced Pharmaceutical Journal. 6(4):111-115.

Okokon JE, Nwafor PA, Noah K. (2011b). Nephroprotective effect of Croton zambesicus root extract against gentimicin-induced kidney injury. Asian Pacific Journal of Tropical Medicine 4: 969 - 972.

Okokon JE, Nwafor PA. (2009a). Antiplasmodial activity of root extract and fractions of Croton zambesicus. Journal of Ethnopharmacology 121: 74-78.

Okokon JE, Nwafor PA. (2009b). Antiulcer and anticonvulsant activities of Croton zambesicus root extract. Pakistan Journal of Pharmaceutical Sciences 22: 384- 390.

Okokon JE, Nwafor PA. (2010). Antiinflammatory, analgesic and antipyretic activities of ethanolic root extract of Croton zambesicus. Pakistan Journal of Pharmaceutical Sciences 23: 383 - 390.

Okokon JE, Udom GJ, Attah G, Bassey AL. (2022c). Alpha amylase and alpha glucosidase inhibitory activities leaf of Croton zambesicus fractions in wistar rats. Journal of Current Biomedical Research.2(2):158-165.

Okokon JE, Anagboso MO, Etim EI, Asor L O. (2023b). Evaluation of inhibitory Effect of Solenostemon monostachyus on alpha amylase and alpha glucosidase enzymes of rats. Journal of Drug Discovery and Research, 1(2), 1-7.

Qaisar MN, Chaudhary BA, Sajid MU, Hussain N. (2014). Evaluation of ?-glucosidase inhibitory activity of dichloromethane and methanol extracts of Croton bonplandianum Baill. Tropical Journal of Pharmaceutical Research. 13 (11): 1833-1836

Saedi E, Ghein, M, Faiz F, Arami M. (2016). Diabetes mellitus and cognitive impairments. World Journal of Diabetes. 7(10). 412.

Srisongkram, T, Waithong S, Thitimetharoch T, Weerapreeyakul N. (2022). Machine Learning and In Vitro Chemical Screening of Potential _-Amylase and _-Glucosidase Inhibitors from Thai Indigenous Plants. Nutrients 14, 267.

Tan D, Song Y, Wang J, Gao C, Qin L, Zhao Y, Lu Y, Yang Z, He Y. (2023). Identification of sesquiterpene glycosides from Dendrobium nobile and their ?-glycosidase and ?-amylase inhibitory activities Technol, Campinas. Food Sci. 43, e99722, 2023

World Health Organization (2011). Glucose analyser. Core medical equipment information. 2011. www.who.int/medical_devices/en/index.html. Assessed on 3rd June, 2019.

World Health Organization 2016. Global Report on Diabetes. Geneva, 2016.




DOI: https://doi.org/10.14421/biomedich.2025.141.529-533

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Copyright (c) 2025 Jude Efiom Okokon, Utibe Anietie Edem, Chinyelu Clementina Osigwe, Ugonma Florence Uwaeme



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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