Antioxidant Activity Test of Ethanol Extract from The Leaves and Bark of Kapur (Dryobalanops aromatica) from Distillation Solid Waste Using the DPPH and ABTS Methods

Nur Maulida Sari, Abdul Rasyid Zarta, Heriad Daud Salusu, Muhammad Fikri Hernandi, Mohammad Ridho Ramadhan, Nur Indah Pragaloka, Rosalinda Kerawing, Puspitasari Muis, Siti Raihanah, Jasmawati Jasmawati, Farida Aryani

Abstract


One of the endemic plants of East Kalimantan, kapur (Dryobalanops aromatica) belongs to the Dipterocarpaceae family and is typically used by the locals for essential oils. Solid waste is only used as a natural fertilizer, while the leaves and bark of Kapur (Dryobalanops aromatica) are often only used for the essential oil extracted. Research on solid waste from distilling kapur (Dryobalanops aromatica) has never been done. This investigation aimed to find the possible concentration of secondary metabolite chemicals and antioxidants in the ethanol extract of solid waste from distilling Kapur leaves and bark (Dryobalanops aromatica). The ethanol extract of Kapur leaves and bark (Dryobalanops aromatica) will undergo phytochemical testing utilizing a qualitative test method to ascertain the presence of secondary metabolite chemicals. The ethanol extract of Kapur leaves and bark (Dryobalanops aromatica) will be tested for antioxidant activity against DPPH and ABTS free radicals using a UV Spectrophotometer. The results of the phytochemical analysis showed that the ethanol extracts from the leaves and bark contained alkaloids, flavonoids and tannin. Triterpenoids are only found in the bark extracts, while saponins and steroids are only found in the leaves extracts. Antioxidant activity of Dryobalanops atomatica extract showed that ethanol extract displayed an ability to inhibit DPPH free radical in both the leaves and bark ethanol extracts, with percentages of 83.24% and 94.91% at 25 ppm concentration, respectively. Antioxidant activity of Dryobalanops atomatica extract showed that ethanol extract could inhibit DPPH free radical in the leaves and bark ethanol extracts, with percentages of 83.24% and 94.91% at 25 ppm concentration, respectively. Antioxidant activity of Dryobalanops atomatica extract showed that ethanol extract display an ability to inhibit ABTS free radical with a percentage of 84.23% at 50 ppm concentration in the leaves extracts, followed by 82.62% percentage of inhibition at 100 ppm concentration. According to the findings of the study, post-distillation solid waste from Dryobalanops aromatica leaves and bark had the potential to develop as a natural antioxidant.

Keywords


ABTS; DPPH; Dryobalanops aromatica; Kalimantan Timur; Phytochemical

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References


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DOI: https://doi.org/10.14421/biomedich.2025.141.381-387

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Copyright (c) 2025 Nur Maulida Sari, Abdul Rasyid Zarta, Heriad Daud Salusu, Muhammad Fikri Hernandi, Mohammad Ridho Ramadhan, Nur Indah Pragaloka, Rosalinda Kerawing, Puspitasari Muis, Siti Raihanah, Jasmawati Jasmawati, Farida Aryani



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
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