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Effectiveness of Nanoemulsion Formulated with Citronella and Garlic Essential Oils against Fall Armyworm (Spodoptera frugiperda) on Maize Plants
Abstract
Fall armyworm (Spodoptera frugiperda J.E Smith) is a major invasive pest that threatens maize production in Indonesia. This study aimed to evaluate the activity of nanoemulsions of citronella and garlic essential oils, both individually and in combinations at ratios of 1:1, 2:1, and 1:2, against third-instar larvae of S. frugiperda. The essential oils were obtained by steam distillation and formulated into nanoemulsions using Tween 80. Toxicity was assessed via contact assays and analyzed with POLO-PC software to determine LC50 and LC95 values. The citronella–garlic ratio of 2:1 was the most promising, yielding the lowest LC50 (0.1 % at 96 hours) and consistently exhibiting strong synergistic effects at the LC50 level compared with the 1:1 and 1:2 ratios, which more frequently showed additive or antagonistic interactions. The citronella nanoemulsion displayed the smallest particle size (97.8 nm) but the highest polydispersity index (PI) (0.406), indicating lower physical stability. In contrast, the garlic nanoemulsion exhibited a particle size of 185.9 nm with a PI of 0.270, reflecting a more stable system. The citronella–garlic combination at a 2:1 ratio produced the largest particle size (204.9 nm) but the lowest PI (0.239), suggesting superior stability and a more uniform particle distribution. These findings support the potential of nanoemulsified essential oils as eco-friendly botanical insecticides.
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Ashauer, R., Agatz, A., Albert, C., Ducrot, V., Galic, N., Hendriks, J., Jager, T., Kretschmann, A., O’Connor, I., Rubach, M. N., Nyman, A. M., Schmitt, W., Stadnicka, J., van den Brink, P. J., & Preuss, T. G. (2011). Toxicokinetic-toxicodynamic modeling of quantal and graded sublethal endpoints: A brief discussion of concepts. Environmental Toxicology and Chemistry, 30(11), 2519–2524. https://doi.org/10.1002/etc.639
Bayala, B., Coulibaly, A. Y., Djigma, F. W., Nagalo, B. M., Baron, S., Figueredo, G., Lobaccaro, J. M. A., & Simpore, J. (2020). Chemical composition, antioxidant, anti-inflammatory and antiproliferative activities of the essential oil of Cymbopogon nardus, a plant used in traditional medicine. Biomolecular Concepts, 11(1), 86–96. https://doi.org/10.1515/bmc-2020-0007
Bhatwalkar, S. B., Mondal, R., Krishna, S. B. N., Adam, J. K., Govender, P., & Anupam, R. (2021). Antibacterial Properties of Organosulfur Compounds of Garlic (Allium sativum). Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb.2021.613077
Chaubey, M. K. (2011). Fumigant toxicity of essential oils against rice weevil Sitophilus oryzae L. (Coleoptera: Curculionidae). Journal of Biological Sciences, 11(6), 411–416. https://doi.org/10.3923/jbs.2011.411.416
Chou, T.-C., & Talalay, P. (1984). Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Advances in Enzyme Regulation, 22, 27–55. https://doi.org/10.1016/0065-2571(84)90007-4
Dono, D., Maryani, L., & Yulia, E. (2019). The Effect of Neem, Citronella, Castor Oil, and Their Mixtures against Crocidolomia pavonana F. (Lepidoptera: Crambidae). CROPSAVER - Journal of Plant Protection, 2(2), 67. https://doi.org/10.24198/cropsaver.v2i2.24063
Du, E. J., Ahn, T. J., Choi, M. S., Kwon, I., Kim, H. W., Kwon, J. Y., & Kang, K. J. (2015). The Mosquito Repellent Citronellal Directly Potentiates Drosophila TRPA1, Facilitating Feeding Suppression. Molecules and Cells, 38(10), 911–917. https://doi.org/10.14348/MOLCELLS.2015.0215
FAO, & CABI. (2019). Fall Armyworm Field Handbook. 1–36. www.cabi.org/fallarmyworm%0Awww.fao.org/fall-armyworm/en
Firmansyah, E., Hidayat, C., Roosda, A. A., Pradana, A. N. F. A., & Soelistijono, R. (2023). The effectiveness of garlic extract against Spodoptera litura on Chili. Kultivasi, 22(3). https://doi.org/10.24198/kultivasi.v22i3.50318
Gharsan, F. N. (2024). Bioactivity of Plant Nanoemulsions against Stored-Product Insects (Order Coleoptera): A Review. Journal of Entomological Science, 59(4), 355–365. https://doi.org/10.18474/JES23-84
Giuliano, G., Campolo, O., Forte, G., Urbaneja, A., Pérez-Hedo, M., Latella, I., Palmeri, V., & Giunti, G. (2024). Insecticidal Activity of Allium sativum Essential Oil-Based Nanoemulsion against Spodoptera littoralis. Insects, 15(7). https://doi.org/10.3390/insects15070476
Gnankiné, O., & Bassolé, I. L. H. N. (2017). Essential oils as an alternative to pyrethroids’ resistance against anopheles species complex giles (Diptera: Culicidae). In Molecules (Vol. 22, Issue 10). MDPI AG. https://doi.org/10.3390/molecules22101321
Hasheminya, S.-M., & Dehghannya, J. (2020). Green synthesis and characterization of copper nanoparticles using Eryngium caucasicum Trautv aqueous extracts and its antioxidant and antimicrobial properties. Particulate Science and Technology, 38(8), 1019–1026. https://doi.org/10.1080/02726351.2019.1658664
Hasrianda, E. F., & Setiarto, R. H. B. (2022). Potensi Rekayasa Genetik Bawang Putih terhadap Kandungan Senyawa Komponen Bioaktif Allicin dan Kajian Sifat Fungsionalnya. Jurnal Pangan, 31(2), 167–190. https://doi.org/10.33964/jp.v31i2.586
Jiang, Z. L., Akhtar, Y., Zhang, X., Bradbury, R., & Isman, M. B. (2012). Insecticidal and feeding deterrent activities of essential oils in the cabbage looper, Trichoplusia ni (Lepidoptera: Noctuidae). Journal of Applied Entomology, 136(3), 191–202. https://doi.org/10.1111/j.1439-0418.2010.01587.x
Juniarti, Osmeli, D., & . Y. (2010). Kandungan Senyawa Kimia, Uji Toksisitas (Brine Shrimp Lethality Test) Dan Antioksidan (1,1-Diphenyl-2-Pikrilhydrazyl) Dari Ekstrak Daun Saga (Abrus Precatorius L.). Makara Journal of Science, 13(1). https://doi.org/10.7454/mss.v13i1.378
Kotambunan, O. F., Salaki, C. L., & Tarore, D. (2020). Efektivitas Ekstrak Serai Wangi (Cymbopogon nardus) sebagai Insektisida Nabati untuk Pengendalian Larva Crocidolomia pavonana Zell. pada Tanaman Kubis. Jurnal Enfit : Entomologi Dan Fitopatologi, 1(1), 1. https://doi.org/10.35791/jef.v1i1.27161
Nerio, L. S., Olivero-Verbel, J., & Stashenko, E. (2010). Repellent activity of essential oils: A review. Bioresource Technology, 101(1), 372–378. https://doi.org/10.1016/j.biortech.2009.07.048
Noor, E., Harmi, L., Maddu, A., & Yusron, M. (2015). Fabrication of nanogingerol by combining phase inversion composition and temperature. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(1), 38–47.
Nuryanti, N. S. P. (2019). Pengembangan Formulasi Nanoemulsi Insektisida Yang Mengandung Campuran Ekstrak Piper Retrofractum Dan Tagetes Erecta Untuk Pengendalia Wereng Batang Cokelat (Nilaparvata Lugens). Institut Pertanian Bogor.
Nuryanti, N. S. P., Martono, E., Ratna, E. S., & Dadang. (2018). Characteristics And Toxicity Of Nanoemulsion Formulation Of Piper Retrofractum And Tagetes Erecta Extract Mixtures. Journal of Tropical Plant Pests and Diseases, 18(1), 1–11. https://doi.org/10.23960/j.hptt.1181-11
Nuryanti, N. S. P., Yuriansyah, & Budiarti, L. (2022). Toxicity and Compatibility of Botanical Insecticide from Clove (Syzygium Aromaticum), Lime (Citrus Aurantifolia) and Garlic (Allium Sativum) Essential oil Against Callasobruchus Chinensis L. IOP Conference Series: Earth and Environmental Science, 1012(1). https://doi.org/10.1088/1755-1315/1012/1/012036
Ostertag, F., Weiss, J., & McClements, D. J. (2012). Low-energy formation of edible nanoemulsions: Factors influencing droplet size produced by emulsion phase inversion. Journal of Colloid and Interface Science, 388(1), 95–102. https://doi.org/10.1016/j.jcis.2012.07.089
Plata-Rueda, A., Martínez, L. C., Santos, M. H. Dos, Fernandes, F. L., Wilcken, C. F., Soares, M. A., Serrão, J. E., & Zanuncio, J. C. (2017). Insecticidal activity of garlic essential oil and their constituents against the mealworm beetle, Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae). Scientific Reports, 7. https://doi.org/10.1038/srep46406
Puspa, O. E., Syahbanu, I., & Wibowo, M. A. (2017). Uji Fitokimia dan Toksisitas Minyak Atsiri Daun Pala (Myristica fragans houtt) dari Pulau Lemukutan. Jurnal Kimia Khatulistiwa, 6(2), 1–6.
Russianzi, W., & Prijono, D. (2019). Aktivitas Sinergistik Campuran Ekstrak Buah Piper aduncum dan Tiga Jenis Insektisida terhadap Ulat Plutella xylostella. Cropsaver, 1(1), 7–14. https://doi.org/10.24198/cs.v1i1.21352
Saleh, S., Pasaru, F., Toana, M. H., Hasriyanty, Syair, M., & Wangi, A. S. (2023). Antifeedant and toxicity effect of citronella essential oil against Spodoptera frugiperda J.E. Smith larvae. IOP Conference Series: Earth and Environmental Science, 1253(1). https://doi.org/10.1088/1755-1315/1253/1/012006
Sarri, K., Mourouzidou, S., Ntalli, N., & Monokrousos, N. (2024). Recent Advances and Developments in the Nematicidal Activity of Essential Oils and Their Components against Root-Knot Nematodes. Agronomy, 14(1). https://doi.org/10.3390/agronomy14010213
Shang, A., Cao, S. Y., Xu, X. Y., Gan, R. Y., Tang, G. Y., Corke, H., Mavumengwana, V., & Li, H. Bin. (2019). Bioactive compounds and biological functions of garlic (allium sativum L.). In Foods (Vol. 8, Issue 7). MDPI Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/foods8070246
Soetomo. (1987). Bertanam Bawang. Bali Pustaka Karya Baru.
Tak, J.-H., & Yoon, J. (2022). Synergistic modes of interaction between the plant essential oils and the respiratory blocker chlorfenapyr. Pesticide Biochemistry and Physiology, 188, 105274. https://doi.org/10.1016/j.pestbp.2022.105274
Usseglio, V. L., Dambolena, J. S., & Zunino, M. P. (2023). Can Essential Oils Be a Natural Alternative for the Control of Spodoptera frugiperda? A Review of Toxicity Methods and Their Modes of Action. In Plants (Vol. 12, Issue 1). MDPI. https://doi.org/10.3390/plants12010003
Wulansari, R., Hidayat, Y., & Dono, D. (2022). Aktivitas Insektisida Campuran Minyak Mimba (Azadirachta indica) dan Minyak Jarak Kepyar (Ricinus communis) terhadap Spodoptera frugiperda Insecticide Activity Mixture Neem Oil (Azadirachta indica) and Castor Oil (Ricinus communis) Against Spodoptera Frug. Jurnal Agrikultura, 2021(3), 207–218.
Yakoubi, R., Megateli, S., Hadj Sadok, T., Bensouici, C., & Ba?ci, E. (2021). A synergistic interactions of Algerian essential oils of Laurus nobilis L., Lavandula stoechas L. and Mentha pulegium L. on anticholinesterase and antioxidant activities. Biocatalysis and Agricultural Biotechnology, 31. https://doi.org/10.1016/j.bcab.2020.101891
Yu, L., Li, C., Xu, J., Hao, J., & Sun, D. (2012). Highly stable concentrated nanoemulsions by the phase inversion composition method at elevated temperature. Langmuir, 28(41), 14547–14552. https://doi.org/10.1021/la302995a
DOI: https://doi.org/10.14421/biomedich.2026.151.1595-1603
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