Email this article 
Antioxidant and Antibacterial Properties of Methanol Extract of Gletang Flower (Tridax procumbens)
Abstract
In a previous study, the methanol extract of Tridax procumbens flowers (commonly known as gletang) at high concentrations was reported to exhibit strong antibacterial activity against Streptococcus mutans and Enterococcus faecalis. This study aimed to examine the secondary metabolite content, antioxidant activity, and antibacterial activity of the methanol extract of gletang flowers. The antioxidant activity was evaluated using two methods, namely DPPH and ABTS assays. The antibacterial activity was assessed using the Kirby-Bauer method at concentrations of 1000, 5000, and 10000 ppm against Staphylococcus aureus, Escherichia coli, Streptococcus mutans, and Enterococcus faecalis. Phytochemical screening revealed that the methanol extract of gletang flowers contains phenolic compounds, flavonoids, alkaloids, and triterpenoids. The antioxidant activity tests showed very strong activity in both DPPH and ABTS assays, with IC50 values of 6.8478 ± 1.335 and 12.8608 ± 0.579 ppm, respectively. The antibacterial activity tests showed that the methanol extract of gletang flowers exhibited strong activity against E. faecalis at concentrations of 5000 and 10000 ppm, with inhibition zone diameters of 3.00 ± 0.14 and 3.20 ± 0.14 mm, respectively.
Keywords
Full Text:
PDFReferences
Aldayel, M. F. (2023). Potential antibacterial and antioxidant inhibitory activities of Silybum marianum mediated biosynthesised He-Ne laser. Saudi Journal of Biological Sciences, 30(11), 103795. https://doi.org/10.1016/j.sjbs.2023.103795
Alkowni, R., Jaradat, N., & Fares, S. (2023). Total phenol, flavonoids, and tannin contents, antimicrobial, antioxidant, vital digestion enzymes inhibitory and cytotoxic activities of Verbascum fruticulosum. European Journal of Integrative Medicine, 60(November 2022), 102256. https://doi.org/10.1016/j.eujim.2023.102256
Andriana, Y., Xuan, T. D., Quy, T. N., Minh, T. N., Van, T. M., & Viet, T. D. (2019). Antihyperuricemia, antioxidant, and antibacterial activities of tridax procumbens L. Foods, 8(1), 1–12. https://doi.org/10.3390/foods8010021
Bialangi, N., Musa, W. J. A., & Situmeang, B. (2024). Metabolite Profiling of Potential Fraction From Ethyl Acetate Extract of Ziziphus mauritiana Leaves by LC-MS/MS Analysis. Karbala International Journal of Modern Science, 10(4), 542–548. https://doi.org/10.33640/2405-609X.3371
Dattaray, D. (2022). Traditional Uses and Pharmacology of Plant Tridax procumbens: A Review. Sys Rev Pharm, 13(7), 476–482. https://doi.org/10.31858/0975-8453.13.5.511-517
Idowu, D. O., Aiyelaagbe, O. O., & Idowu, P. A. (2023). Chemical composition and biological activities of volatile oil of the stem of Dombeya buettneri K. Schum. (Sterculiaceae). Scientific African, 20, 1–10. https://doi.org/10.1016/j.sciaf.2023.e01624
Ingole, V. V., Mhaske, P. C., & Katade, S. R. (2022). Phytochemistry and pharmacological aspects of Tridax procumbens (L.): A systematic and comprehensive review. Phytomedicine Plus, 2(1), 100199. https://doi.org/10.1016/j.phyplu.2021.100199
Jiangseubchatveera, N., Saechan, C., Petchsomrit, A., Treeyaprasert, T., Leelakanok, N., & Prompanya, C. (2023). Phytochemicals and Antioxidant Activities of Red Oak, Red Coral and Butterhead. Tropical Life Sciences Research, 34(1), 1–17. https://doi.org/10.21315/tlsr2023.34.1.1
Kabré, P., Ouattara, L., Sanou, Y., Ouédraogo, R. J., Ouoba, P., Zanté, A. A., Zoungo, D., Somda, M. B., & Ouédraogo, G. A. (2023). Comparative study of polyphenols, flavonoids content, antioxidant and antidiabetic activities of Lophira lanceolata Tiegh.ex Keay (Ochnaceae) extracts. Scientific African, 22(October). https://doi.org/10.1016/j.sciaf.2023.e01922
Liu, L., Chen, J., Chang, X., Qin, J., Lai, H., & Zhang, X. (2023). Phytochemical profiles and bioactivities of Parnassia palustris L. European Journal of Integrative Medicine, 57(September 2022), 102207. https://doi.org/10.1016/j.eujim.2022.102207
Pradana, A. F., Fauji, F. R., & Farlina, I. (2024). The potential of methanol extract nanoemulsion from gletang flower ( Tridax procumbens ) as an antibacterial agent against pathogenic bacteria. 0–4.
Satari, M. H., Situmeang, B., Yuda, I. P., & Kurnia, D. (2019). Antibacterial Diterpenoid Against Pathogenic Oral Bacteria of Streptococcus Mutans ATCC 25175 Isolated From Sarang Semut (Myrmecodia Pendans). Jurnal Kimia Valensi, 5(2), 218–223. https://doi.org/10.15408/jkv.v5i2.8864
Situmeang, B., Primawati, J. S., Oktafiani, I., Musa, W. J. A., & Kilo, A. K. (2025). Antioxidant and Cytotoxic Potential of Ethyl Acetate Fraction of Gandaria Stem Bark ( Bouea macrophylla ) Against MCF-7 Cell Line. 14(1), 345–349. https://doi.org/10.14421/biomedich.2025.141.345-349
Situmeang, B., Swasono, R. T., & Raharjo, T. J. (2025). Evaluation of phytochemical composition, antioxidant, cytotoxic and in silico studies of ethyl acetate fractions of Tristaniopsis merguensis leaves. Toxicology Reports, 14(January), 101911. https://doi.org/10.1016/j.toxrep.2025.101911
Widyawati, Arma, U., Fadriyanti, O., Situmeang, B., & Silaban, S. (2022). Antibacterial Activity Test of Different Parts of Gletang (Tridax Procumbens) From West Sumatera, Indonesia. Rasayan Journal of Chemistry, 15(4), 2382–2386. https://doi.org/10.31788/RJC.2022.1547084
Wijayanti, S., Pradana, A. F., Situmeang, B., Prastiwi, D. A., & Musa, W. J. A. (2025). Microemulsion of methanol extract of Tridax procumbens flower and its antibacterial activity against Streptococcus mutans and Enterococcus faecalis. Jurnal Beta Kimia, 5(1), 56–61. https://doi.org/10.35508/jbk.v5i1.21189
DOI: https://doi.org/10.14421/biomedich.2025.142.1085-1090
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Boima Situmeang, Weny JA Musa, Nurhayati Bialangi, Sriwijayanti, Holisha Widiyanto, Dian Susvira
Biology, Medicine, & Natural Product Chemistry |
|
